Background: In C. elegans, single nucleotide polymorphisms (SNPs) can function as silent genetic markers, with applications ranging from classical two- and three-factor mapping to measuring recombination across whole chromosomes. Results: Here, we describe a set of 48 primer pairs that flank SNPs evenly spaced across the C. elegans genome and that work under identical PCR conditions. Each SNP in this set alters a DraI site, enabling rapid and parallel scoring. We describe a procedure using these reagents to quickly and reliably map mutations. We show that these techniques correctly map a known gene, dpy-5. We then use these techniques to map mutations in an uncharacterized strain, and show that its behavioral phenotype can be simultaneously mapped to three loci. Conclusion: Together, the reagents and methods described represent a significant advance in the accurate, rapid and inexpensive mapping of genes in C. elegans. © 2005 Davis et al; licensee BioMed Central Ltd.
CITATION STYLE
Davis, M. W., Hammarlund, M., Harrach, T., Hullett, P., Olsen, S., & Jorgensen, E. M. (2005). Rapid single nucleotide polymophism mapping in C. elegans. BMC Genomics, 6. https://doi.org/10.1186/1471-2164-6-118
Mendeley helps you to discover research relevant for your work.