Histopathology requires the expertise of specialists to diagnose morphological features of cells and tissues. Raman imaging can provide additional biochemical information to benefit histological disease diagnosis. Using a dietary model of nonalcoholic fatty liver disease in rats, we combine Raman imaging with machine learning and information theory to evaluate cellular-level information in liver tissue samples. After increasing signal-to-noise ratio in the Raman images through superpixel segmentation, we extract biochemically distinct regions within liver tissues, allowing for quantification of characteristic biochemical components such as vitamin A and lipids. Armed with microscopic information about the biochemical composition of the liver tissues, we group tissues having similar composition, providing a descriptor enabling inference of tissue states, contributing valuable information to histological inspection.
CITATION STYLE
Helal, K. M., Taylor, J. N., Cahyadi, H., Okajima, A., Tabata, K., Itoh, Y., … Komatsuzaki, T. (2019). Raman spectroscopic histology using machine learning for nonalcoholic fatty liver disease. FEBS Letters, 593(18), 2535–2544. https://doi.org/10.1002/1873-3468.13520
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