Role of sweat in accumulation of orally administered griseofulvin in skin

Abstract

Griseofulvin, an orally effective antimicrobial agent, appears in the stratum corneum within 4-8 hr after oral administration. Griseofulvin distribution was found to be highest in the outermost layers of the stratum corneum (level I, 20.8±1.5 ng/mg) and lowest inside (level II, 10.0±1.5; level III, 7.5±2.2 ng/mg). In order to study the precise mechanism of griseofulvin transfer to stratum corneum, the role of sweat in the accumulation of griseofulvin was considered. Heat induced total body sweating decreased the mean stratum corneum concentration of griseofulvin by 55%, and 200-300 ng of griseofulvin accumulated per ml of sweat. A silicone hydrophobic resin was used to differentiate between 'wash off' and carrier properties of sweat for griseofulvin. Prevention of transepidermal water and sweat loss by topical application of formaldehyde releasing cream to one palm, occlusion by a 2 x 2 cm patch on one arm, and wearing a rubber glove for 24 hr showed a lower griseofulvin concentration when compared to control areas in the same subjects. The results of the gloved hand experiment show that a complete equilibrium is established at all 3 levels of stratum corneum, thereby removing the reversed gradient. These results support the hypothesis that a 'wick effect' is responsible for the observed reversed drug gradient within the stratum corneum. The results of the experiments suggest that sweat and transepidermal fluid loss play an important role in griseofulvin transfer in stratum corneum.