A fast and accurate algorithm for the quantification of peptides from mass spectrometry data

Abstract

Liquid chromatography combined with mass spectrometry (LC-MS) has become the prevalent technology in high-throughput proteomics research. One of the aims of this discipline is to obtain accurate quantitative information about all proteins and peptides in a biological sample. Due to size and complexity of the data generated in these experiments, this problem remains a challenging task requiring sophisticated and efficient computational tools. We propose an algorithm that can quantify even low abundance peptides from LC-MS data. Our approach is flexible and can be applied to preprocessed and raw instrument data. It is based on a combination of the sweep line paradigm with a novel wavelet function tailored to detect isotopic patterns. We evaluate our technique on several data sets of varying complexity and show that we are able to rapidly quantify peptides with high accuracy in a sound algorithmic framework. © Springer-Verlag Berlin Heidelberg 2007.