Secretion of oxytocin in pregnant and parturient cows: Corpus luteum may contribute to plasma oxytocin at term

Abstract

Plasma oxytocin (OT) concentrations were determined in 14 late-pregnant and parturient Angus-Hereford cows. Jugular and utero-ovarian veins were cannulated for simultaneous withdrawal of blood samples. Samples were collected at 10-min intervals for 6 h once weekly beginning 60-14 days before the date of expected delivery (group 1), or daily 3-7 days before the due date (group 2). In a third group, samples were collected at 15-min intervals every other day for 12 h beginning 1 wk before calving. Basal levels of OT were low, the overall mean for both veins was 0.46 ± 0.03 μU/ml until a week before parturition, and then increased to 0.77 ± 0.1 μU/ml (P < 0.02). Spurts of OT occurred intermittently on all days. Interpeak intervals averaged 71.0 ± 10.7 min until Day -14, and from Day -14 to Day -1 the intervals were 44.0 ± 5.3 min (P < 0.05). From Day -60 to Day -25 the amplitudes of OT peaks were low and similar in both veins (mean 1.37 ± 0.1 μU/ml). From Day -14 to Day -1 the peak amplitudes were 3.6 ± 0.4 μU/ml on average (P < 0.02). During the last 2 wk the utero-ovarian peak of OT was frequently higher than the peripheral peak. In addition, a number of spurts were observed in the utero-ovarian vein only (solo peaks). On the day of parturition during the first stage of labor, peak amplitudes had increased to 7.3 ± 2.0 μU/ml, and the interpeak intervals had become shorter than before labor (mean 25.1 ± 2.6 min). A large surge of OT initiated the expulsive stage of labor. Basal levels rose to 43.1 ± 16 μU/ml and 38.7 ± 12.6 μU/ml, and peak levels to 77.4 ± 19.1 μU/ml and 91.6 ± 21 μU/ml in the jugular and utero-ovarian veins, respectively. Interpeak intervals had decreased to 17.2 ± 3.3 min (P < 0.05). Oxytocin levels remained high after delivery of the calf until the placenta was expelled. The posterior pituitary was the source of circulating OT during most of gestation and labor, but the solo peaks observed during late gestation in the utero-ovarian vein were probably of luteal origin or possibly of caruncular origin, because near term, both tissues express OT mRNA. Fetal posterior pituitary is another possible source for these peaks. Our conclusions are that during bovine pregnancy, low amplitude spurts of OT are secreted intermittently; near term, both the frequency and peak amplitude of the spurts increase; and during labor, a dramatic increase in plasma OT precedes the expulsion of the calf. The main source of OT is the posterior pituitary, but near term, a utero-ovarian source secretes additional OT into the systemic circulation.