Indirect Spectrophotometric Determination of Paracetamol Via Decolorization of Eriochrom Black-T With N-Bromosuccinimide

Abstract

A simple, precise and sensitive indirect spectrophotometric method is described for the assay of Paracetamol in its pure form and pharmaceutical formulations in the aqueous medium. The method is based on the oxidation of the Paracetamol with an excess of N-Bromosuccinimide (NBS) in alkaline medium and the residual oxidizing agent bleaches the purple-colored Eriochrome black-T (EBT) to colorless species which is measured at 516 nm at room temperature. Calibration graph is linear over 0.5-9 µg mL-1 and molar absorptivity is 3.7×10 4 L.mol-1 .cm-1. The detection and quantification limits were 0.068 and 0.229 µgmL-1 respectively. The accuracy (Average recovery %) is 98.57, and Precision (RSD) is ≤ 1.5. No interference effect has been observed from the excipients that exist in drug formulations. The method has been applied successfully in the determination of the Paracetamol in its commercial formulations (injection, syrup, and tablet), and compared favorably with other spectrophotometric methods used different reagents. The reaction mechanism for the oxidation of Paracetamol and EBT was postulated. ‫اسيتامول‬ ‫للبار‬ ‫المباشر‬ ‫غير‬ ‫الطيفي‬ ‫التقدير‬ ‫ب‬ ‫قصر‬ ‫لون‬ ‫ال‬ ‫بالك‬ ‫ريوكروم‬-T ‫اسطة‬ ‫بو‬ N-‫بروموسكسينئميد‬ ‫الجبوري‬ ‫جميل‬ ‫اء‬ ‫زهر‬ 1 * ، ‫الصبحة‬ ‫نجم‬ ‫ضياء‬ 2 1 ‫و‬ 2 ‫اق‬ ‫العر‬ ،‫الموصل‬ ،‫الموصل‬ ‫جامعة‬ ،‫الصرفة‬ ‫للعلوم‬ ‫بية‬ ‫التر‬ ‫كلية‬ ،‫الكيمياء‬ ‫قسم‬ ‫الخالصة‬ : ‫اته‬ ‫مستحضر‬ ‫وفي‬ ‫النقية‬ ‫بصيغته‬ ‫اسيتامول‬ ‫البار‬ ‫اء‬ ‫دو‬ ‫لتقدير‬ ‫وحساسة‬ ‫ومضبوطة‬ ‫بسيطة‬ ‫ة‬ ‫مباشر‬ ‫غير‬ ‫طيفية‬ ‫يقة‬ ‫طر‬ ‫وصفت‬ ‫ا‬ ‫بو‬ ‫اسيتامول‬ ‫البار‬ ‫أكسدة‬ ‫على‬ ‫يقة‬ ‫الطر‬ ‫اعتمدت‬ ‫حيث‬ .‫المائي‬ ‫الوسط‬ ‫في‬ ‫ائية‬ ‫الدو‬ ‫من‬ ‫يادة‬ ‫ز‬ ‫سطة‬ N-،‫القاعدي‬ ‫الوسط‬ ‫في‬ ‫بروموسكسينئميد‬ ‫بالك‬ ‫يوكروم‬ ‫األر‬ ‫صبغة‬ ‫قصر‬ ‫على‬ ‫يعمل‬ ‫المؤكسد‬ ‫العامل‬ ‫من‬ ‫الفائض‬ ‫و‬-T ‫الموجي‬ ‫الطول‬ ‫عند‬ ‫الصبغة‬ ‫امتصاص‬ ‫وقياس‬ 516 ‫نانوميتر‬ ‫التركيز‬ ‫مدى‬ ‫في‬ ‫خطي‬ ‫القياسي‬ ‫المنحنى‬ ‫ان‬ ‫وجد‬ ‫لقد‬ .‫المختبر‬ ‫ة‬ ‫ار‬ ‫حر‬ ‫درجة‬ ‫عند‬ ‫وذلك‬ 0.5-9.0 ‫ام/مل‬ ‫مايكروغر‬ ‫االمتصاصية‬ ‫وبلغت‬ ،‫لتر‬