A panel of four murine monoclonal antibodies showing species specificity for the yeast phase of the pathogenic dimorphic fungus Paracoccidioides brasiliensis was produced by using a modification of the standard monoclonal antibody technology. This involved the use of the immunosuppressive drug cyclophosphamide to suppress the immune response of test animals to fungi showing cross-reactivity, i.e., to Histoplasma capsulatum. One monoclonal antibody, P4, which had a high titer by enzyme-linked immunosorbent assay, was shown to recognize a linear antigenic epitope of P. brasiliensis at a molecular size of 70,000 to 75,000 daltons by Western blot (immunoblot) analysis. The potential use of these monoclonal antibodies, which are the first species-specific probes to P. brasiliensis that have been produced, in the field of serodiagnosis is discussed.
CITATION STYLE
Figueroa, J. I., Hamilton, A. J., Bartholomew, M. A., Harada, T., Fenelon, L., & Hay, R. J. (1990). Preparation of species-specific murine monoclonal antibodies against the yeast phase of Paracoccidioides brasiliensis. Journal of Clinical Microbiology, 28(8), 1766–1769. https://doi.org/10.1128/jcm.28.8.1766-1769.1990
Mendeley helps you to discover research relevant for your work.