Single-laboratory validation of hplc-elsd method for quantitative analysis of plant glucosylceramides

Abstract

Plant glucosylceramides (GlcCer) are currently used as functional materials in supplements, general foods, and drinks for skin care. However, the routine analytical method for quantification of the functional lipid has not yet been established because it is difficult to obtain analytical standard material. In this study, we carried out singlelaboratory validation of the high-performance liquid chromatography/evaporative light-scattering detection (HPLCELSD) method for expansion of the matrix which has already been established. Performance characteristics such as selectivity, linearity, trueness (recovery), repeatability, and intermediate precision were examined. The reported analytical protocol, which was developed for the quantification of wheat GlcCer, was shown to be also applicable for different matrices such as rice and corn. Moreover, judging from these results together with published data, it was assumed that the method could be extended to other matrices by using the standard curves of individual GlcCer prepared from the same source material as the target matrices.