Anode based on alcohol dehydrogenase enzyme and Titanium dioxide nanotubes for photocatalytic microfluidic device.

Abstract

Alcohol dehydrogenase enzyme (ADH) has been used for the development of bioanodes for the ethanol oxidation reaction. In this work, the immobilization of this enzyme was carried out using titanium dioxide nanotubes for the development of photoelectrodes for an air-breathing microfluidic device. TiO2 nanotubes (TNT) have excellent properties such as pH resistance, superior mechanical strength, good biocompatibility making them great candidates for the process of immobilization of ADH. TNT were fabricated by electrochemical anodic oxidation on Ti foils. Then enzyme alcohol dehydrogenase was immobilized and the electrode was evaluated in the microfluidic fuel cell using an inorganic cathode based on commercial Pt / C, obtaining a good performance when was exposed under ultraviolet light.