Compared with luciferase which is widely used as a reporter for circadian rhythms in Arabidopsis thaliana, available fluorescent markers are generally too stable to allow circadian oscillations to be measured. However, we have developed a technique to use the nuclear localization of circadian-controlled transcription factors fused to a fluorescent reporter as a means of measuring circadian rhythms. This technique has the advantage of being suitable for analyzing rhythms at the level of individual cells and in living plants.
CITATION STYLE
Shor, E., Hassidim, M., & Green, R. M. (2014). The Use of Fluorescent Proteins to Analyze Circadian Rhythms. Methods in Molecular Biology, 1158, 209–213. https://doi.org/10.1007/978-1-4939-0700-7_14
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