Microbial-based biotherapeutics that are produced in Escherichia coli (E. coli) can be generated intracellularly in the form of inclusion bodies (IBs) or in soluble active form in periplasmic space or extracellularly. Overexpression of these biotherapeutics in E. coli leads to formation of insoluble aggregates called inclusion bodies. These IBs contain misfolded and inactive form of proteins which need to be refolded to obtain a functionally active form of proteins. Here, we discuss refolding of E. coli-based recombinant human granulocyte colony-stimulating factor (GCSF), expressed as IBs, and highlight some of the key features associated with the refolding kinetic reaction.
CITATION STYLE
Sharma, R., Anupa, A., & Rathore, A. S. (2023). Refolding of Proteins Expressed as Inclusion Bodies in E. coli. Methods in Molecular Biology (Clifton, N.J.), 2617, 201–208. https://doi.org/10.1007/978-1-0716-2930-7_14
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