Electrophoretic mobility shift assays for the analysis of DNA-protein interactions

Abstract

Electromobility shift assay is a simple, efficient, and rapid method for the study of specific DNA-protein interactions. It relies on the reduction in the electrophoretic mobility conferred to a DNA fragment by an interacting protein. The technique is suitable to qualitative, quantitative, and kinetic analyses. It can also be used to analyze conformational changes. © 2009 Humana Press, a part of Springer Science+Business Media, LLC.