Large‐Scale Preparation of Soluble Adenovirus Hexon, Penton and Fiber Antigens in Highly Purified Form

Abstract

A three‐step mild procedure is described, that permits isolation of an adenovirus type‐2 soluble hexon, penton and fiber antigens in an extensively purified form. This procedure comprises ammonium sulfate precipitation, Sephadex ion‐exchange chromatography and adsorption chromatography on an hydroxyapatite column. The final antigen preparations were homogeneous with regard to immunological, electrophoretic and electron microscopic criteria. Copyright © 1973, Wiley Blackwell. All rights reserved