Detection of protein–protein interactions in tobacco BY-2 cells using bimolecular fluorescence complementation

Abstract

Knowledge of protein–protein interactions in the plant cell is invaluable for furthering our understanding of the functions of these proteins. Many of the methods available for the study of these interactions, such as yeast two-hybrid and co-immunoprecipitation assays, rely on in vitro techniques. Here we describe the use of bimolecular fl uorescence complementation for the study of protein–protein interactions in vivo, using simple techniques and accessible materials.