Many bacterial species move toward favorable habitats. The flagellum is one of the most important machines required for the motility in solution and is conserved across a wide range of bacteria. The motility machinery is thought to function efficiently with a similar mechanism in a variety of environmental conditions, as many cells with similar machineries have been isolated from harsh environments. To understand the common mechanism and its diversity, microscopic examination of bacterial movements is a crucial step. Here, we describe a method to characterize the swimming motility of cells in extreme environmental conditions. This microscopy system enables acquisition of high-resolution images under high-pressure conditions. The temperature and oxygen concentration can also be manipulated. In addition, we also describe a method to track the movement of swimming cells using an ImageJ plugin. This enables characterization of the swimming motility of the selected cells.
CITATION STYLE
Nishiyama, M., & Arai, Y. (2017). Tracking the movement of a single prokaryotic cell in extreme environmental conditions. In Methods in Molecular Biology (Vol. 1593, pp. 175–184). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6927-2_13
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