Susceptibility of vertilmicin to modifications by three types of recombinant aminoglycoside-modifying enzymes

Abstract

The susceptibilities of vertilmicin and seven reference aminoglycosides to modifications by six recombinant aminoglycoside-modifying enzymes, AAC(6′)-Ie, APH(2″)-Ia, AAC(6′)-Ie-APH(2″)-Ia, ANT(2″)-Ia, AAC(6′)-Ib, and AAC(6′)-Ib-cr, were studied by coupled spectrophotometric assays in microtiter plates. In comparison to other aminoglycosides, the susceptibility of vertilmicin was 45.8- to 250.0-fold lower for AAC(6′)-Ie acetylation, 39.2- to 116.7-fold lower for AAC(6′)-Ie-APH(2″)-Ia acetylation, and 1.8- to 7.5-fold lower for ANT(2″)-Ia adenylation (except that shown by amikacin) while relatively comparable for AAC(6′)-Ib acetylation, AAC(6′)-Ib-cr acetylation, APH(2″)-Ia phosphorylation, and AAC(6′)-Ie-APH(2″)-Ia phosphorylation. Copyright © 2011, American Society for Microbiology. All Rights Reserved.