Efficient and reliable methods for gene editing are critical for the generation of loss-of-gene function stem cells and genetically modified mice. Here, we outline the application of CRISPR-Cas9 technology for gene editing in mouse embryonic stem cells (mESCs) to generate knockout ESC chimeras for the fast-tracked analysis of gene function. Furthermore, we describe the application of gene editing directly to mouse epiblast stem cells (mEpiSCs) for modelling germ layer differentiation in vitro.
CITATION STYLE
Sibbritt, T., Osteil, P., Fan, X., Sun, J., Salehin, N., Knowles, H., … Tam, P. P. L. (2019). Gene editing of mouse embryonic and epiblast stem cells. In Methods in Molecular Biology (Vol. 1940, pp. 77–95). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9086-3_6
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