Effects of pyridalyl on ATP concentrations in cultured Sf9 cells

Abstract

The effects of pyridalyl and various cytotoxic substances on ATP levels in cultured Sf9 cells were compared. Levels decreased markedly after treatment with the mitochondrial respiration disruptors acequinocyl (10μM), chlorfenapyr (1.0-10μM), fenpyroxymate (0.01-1.0μM), rotenone (0.1-10μM), 2-chlorocar-bonylcyanide phenylhydrazine (0.1-1.0μM) and oligomycin (0.1-10μM), but increased after treatment with 10 μM of anisomycin, a protein synthesis inhibitor. An RNA synthesis inhibitor, 5-fluorouracil (0.1-10μM), did not have any effect. Pyridalyl (0.1-10μM) reduced the ATP content of the cells; however, the rate of the decrease was not rapid. © Pesticide Science Society of Japan.