Sublethal effects of a vapour-active pyrethroid, transfluthrin, on Aedes aegypti and Ae. albopictus (Diptera: Culicidae) fecundity and oviposition behaviour

Abstract

Background: It is assumed that mosquitoes surviving exposure to spatial repellents when attempting to bite a host will not have significant adverse impacts on their downstream biology. Therefore, a critical knowledge gap is understanding the extent to which sublethal exposure to volatile pyrethroids may damage the performance of mosquitoes that survive exposure to vapour-active pyrethroids. To address this, laboratory-reared Aedes aegypti (L.) and Ae. albopictus (Skuse) were exposed to one of three sublethal concentrations of transfluthrin before being offered a blood-meal, after which their survival, fecundity, fertility, and egg-laying behaviour was assessed. Results: Both species expressed reduced skip-oviposition behaviour at all exposures. Both species also suffered a major reduction in viable eggs (50-75% reduction in viable eggs laid). A phenotype where eggs collapsed after laying was observed in Ae. aegypti, and this response increased with exposure concentrations. Dissected females of both species retained 50% or fewer of their eggs, with Ae. albopictus retaining a significant proportion of melanised oocytes following the highest exposure. Conclusions: Our findings suggest that volatile pyrethroids can reduce skip-oviposition, which may improve source reduction outcomes during integrated management. The additional fecundity reduction caused by sublethal exposures to volatile pyrethroids improves our confidence in recommending them for urban vector management. Furthermore, we suggest that volatile pyrethroids should be adapted into delivery methods compatible with mosquito abatement programs.