MiT-383 down-regulates the oncogene CIP2A to influence glioma proliferation and invasion

Abstract

Background: Recent evidence showed cancerous inhibitor of protein phosphatase 2A (CIP2A) plays carcinogenesis roles in several types of human cancer. However, the expression and function of CIP2A in gliomas are unknown. Methods: qRT-PCR, IHC and Western blot were used to evaluate CIP2A expression in glioma tissues and cell lines. The influence of CIP2A on prognosis was analyzed by KM curve and Cox regression. CCK8, clonal formation, transwell and tumor xenograft assays were used to analyze cell proliferation and invasion. The upstream microRNA of CIP2A was verified by luciferase and RIP assays. Results: CIP2A was overexpressed in gliomas and associated with tumor size, WHO grade and postoperative overall survival rate. Depletion of CIP2A inhibited glioma cellular proliferation, invasion and xenograft tumorigenicity. miR-383 could bind to the 3ʹ-UTR of CIP2A and inhibit CIP2A expression by forming an RNA-induced silencing complex with Ago2. Conclusion: CIP2A plays a carcinogenesis role in glioma progression and is one of the potential targets of miR-383.