A recombinant baculovirus, rv-egfp-NK, containing a reporter gene encoding the enhanced green fluorescent protein (EGFP), was used to express nattokinase (NK), a fibrinolytic enzyme, in Spodoptera frugiperda (SF-9) cells. The recombinant protein also included a histidine tag for purification using Ni 2+ resins. The recombinant NK, ∼30 kDa, retained fibrinolytic activity (60 U/ml). The integration of the EGFP expression cassette in the Bac-to-Bac system is thus an effective method for the expression and purification of recombinant NK protein in Spodoptera frugiperda insect cells. © 2007 Springer Science+Business Media B.V.
CITATION STYLE
Li, X., Wang, X., Xiong, S., Zhang, J., Cai, L., & Yang, Y. (2007). Expression and purification of recombinant nattokinase in Spodoptera frugiperda cells. Biotechnology Letters, 29(10), 1459–1464. https://doi.org/10.1007/s10529-007-9426-2
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