Genome-wide study of gene expression, or transcriptome profiling, is critical for our understanding of biological functions, including developmental processes. Recent breakthroughs in high-throughput sequencing technologies have revolutionized gene expression profiling to study the transcriptome at the nucleotide level, which is known as RNA-seq. RNA-seq, also called "whole transcriptome shotgun sequencing," uses next-generation sequencing technologies to sequence cDNA in order to infer a sample's RNA content. Here we describe a detailed bench-ready protocol to generate RNA-seq libraries for high-throughput single-end or pair-end sequencing compatible with the Illumina sequencing platform. © Springer Science+Business Media, New York 2014.
CITATION STYLE
He, J., & Jiao, Y. (2014). Next-generation sequencing applied to flower development: RNA-Seq. Methods in Molecular Biology, 1110, 401–411. https://doi.org/10.1007/978-1-4614-9408-9_23
Mendeley helps you to discover research relevant for your work.