Cytotoxic Effect of Cantigi [Vaccinium varingiaefolium (Blume) Miq.] Extracts on T47D Cells

Abstract

The previous report showed that the leaf extracts of Cantigi (Vaccinium varingiaefolium) had cytotoxic activity on leukemia cells. This study aimed to investigate the activities of the leaf extracts on the T47D cells and the bioactive compounds of the most active extract. The leaves were extracted step by step using hexane, ethyl acetate, and ethanol. The leaf extracts and the positive control cisplatin were examined on T47D cells. Identification of bioactive compounds of the most active extract was using GC-MS and LC-MS. The results showed that the ethyl acetate extract was the most active (IC50 =75.16±3.05 ppm), and the control was 3.94±0.02 ppm. The GC-MS identified two dominant volatile bioactive compounds, namely hexadecanoic acid and octadecanoic acid. Meanwhile, the LC-MS identified three nonvolatile bioactive compounds (ß-sitosterol-3-O-ß-Dglucopyranoside, bufalin, and trametenolic acid). Moreover, some studies reported that those identified bioactive compounds were active on breast cancer cells.