Phenotyping of Light Response on JA-Defective Mutant in Rice

Abstract

Jasmonates are associated with stress responses. However, there are many roles for jasmonates in plant development. One such function is their participation in the regulation of growth and development of rice seedlings, especially of their coleoptile in response to light. Impairment of jasmonate biosynthesis or signaling results in obvious phenotypic effects in seedlings, which can be used for screening homozygous mutants from a heterozygous population for downstream experimental approaches. A procedure for screening mutants under light is described, which can easily be adapted to standard laboratory conditions.