In this work we use metabolomics and 13C-labeling data to refine central metabolic pathways for methane utilization in Methylosinus trichosporium OB3b, a model alphaproteobacterial methanotrophic bacterium. We demonstrate here that similar to non-methane utilizing methylotrophic alphaproteobacteria the core metabolism of the microbe is represented by several tightly connected metabolic cycles, such as the serine pathway, the ethylmalonyl-CoA (EMC) pathway, and the citric acid (TCA) cycle. Both in silico estimations and stable isotope labeling experiments combined with single cell (NanoSIMS) and bulk biomass analyses indicate that a significantly larger portion of the cell carbon (over 60%) is derived from CO2 in this methanotroph. Our 13C-labeling studies revealed an unusual topology of the assimilatory network in which phosph(enol) pyruvate/pyruvate interconversions are key metabolic switches. A set of additional pathways for carbon fixation are identified and discussed. © 2013 Yang, Matsen, Konopka, Green-Saxena, Clubb, Sadilek, Orphan, Beck and Kalyuzhnaya.
CITATION STYLE
Yang, S., Matsen, J. B., Konopka, M., Green-Saxena, A., Clubb, J., Sadilek, M., … Kalyuzhnaya, M. G. (2013). Global molecular analyses of methane metabolism in methanotrophic alphaproteobacterium, methylosinus trichosporium OB3b. Part II. metabolomics and 13C-labeling study. Frontiers in Microbiology, 4(APR). https://doi.org/10.3389/fmicb.2013.00070
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