Effect of UV irradiation on transduction by coliphage T1

  • Drexler H
  • Kylberg K
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Abstract

Lysates of the virulent coliphage T1 transduce seven markers between strains of Escherichia coli with reproducilble efficiencies which range from 10(-7) to 10(-5). The ability of a UV-irradiated lysate to transduce Arg(+), Str(R), Trp(+), Lac(+), and Pro(+) is 90% and Bio(+) is 99% inactivated by doses which inactivate plaque formation of T1 by six orders of magnitude. A dose of irradiation which causes a 1- to 2-log drop in the titer of T1 stimulated Gal(+) transduction by two- to three-fold; no other marker tested was stimulated. Irradiation causes dislinkage of some co-transducible markers but not others.

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Drexler, H., & Kylberg, K. J. (1975). Effect of UV irradiation on transduction by coliphage T1. Journal of Virology, 16(2), 263–266. https://doi.org/10.1128/jvi.16.2.263-266.1975

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