Sequential analysis of diethylnitrosamine-induced hepatocarcinogenesis in rats

Abstract

In the present study, we examined the sequential changes of diethylnitrosamine (DEN)-induced hepatocarcino-genesis in Wistar rats. After 14weeks of DEN treatment, hyperplastic nodules developed as a consequence of the appearance of renewed hepatocytes, degenerated hepatocytes, oval cells and fibrotic changes. Total bilirubin and alanine aminotransferase levels were significantly higher in the DEN group compared to the control group throughout the experimental period. Our data may prove beneficial to future analyses of chemopreventive compounds during various stages of hepatocarcinogenesis in rats.