An internal 5'-noncoding region required for translation of poliovirus RNA in vitro

Abstract

A truncated poliovirus RNA that contains the entire 5'-noncoding region as well as some capsid protein-coding sequences was produced from cloned cDNA inserted into an SP6 transcription vector and subsequently was translated in a mixed rabbit reticulocyte-HeLa cell lysate. Deletions or modifications of regions of the 5'-noncoding sequences had significant effects upon the efficiency of translation. The presence of a 60-nucleotide sequence located at positions 567 to 627 appeared to be essential for active ribosome binding and translation of this uncapped RNA.