Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology. © 2012 Nature America, Inc. All rights reserved.
CITATION STYLE
Koopmann, R., Cupelli, K., Redecke, L., Nass, K., Deponte, D. P., White, T. A., … Duszenko, M. (2012). In vivo protein crystallization opens new routes in structural biology. Nature Methods, 9(3), 259–262. https://doi.org/10.1038/nmeth.1859
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