Virulance factor of staphylococcus sp. Isolated from subclinical mastitis in ettawa grade goat's milk in sleman regency -yogyakarta

Abstract

Stapphylococcus sp., is bacteria that caused subclinical mastitis in Ettawa Grade (EG) goat. The purpose of this study was to determine virulance factor Stapphylococcus sp., which was isolated from subclinical mastitis EG goat's milk in Sleman regency, Yogyakarta. A total of 7 isolate Stapphylococcus sp., were isolated from subclinical mastitis EG goat's milk were determinated by several virulance factors such as haemolysin, clumping factor, and coagulase. Haemolysin was determinated by culture in blood agar plate and incubated in the temperature of 37°C for 24 hours. Clumping factor was determinated by mixing the rabbit plasma with Stapphylococcus sp., in the glass objects. Coagulase was determinated by mixing the rabbit plasma and broth culture of Stapphylococcus sp. After incubated in the temperature of 37°C for 24 hours in tube, then the gel formation was observed. Haemolytic type ß was yielded from 5 isolate Stapphylococcus sp., whereas 2 isolates were not haemolytic. Clumping factor and coagulase were produced from 2 isolate Stapphylococcus sp. This study showed that not all of Stapphylococcus sp., isolate causing subclinical mastitis in EG goat have virulance factor.