A critical stage in performing gene editing experiments using the CRISPR/Cas9 system is the design of guide RNA (gRNA). In this chapter, we conduct a review of the current gRNA design rules for maximizing on-target Cas9 activity while minimizing off-target activity. In addition, we present some of the currently available computational tools for gRNA activity prediction and assay design.
CITATION STYLE
Hiranniramol, K., Chen, Y., & Wang, X. (2020). CRISPR/Cas9 Guide RNA Design Rules for Predicting Activity. In Methods in Molecular Biology (Vol. 2115, pp. 351–364). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0290-4_19
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