So-called architectural DNA binding proteins such as those of the HMGB-box family induce DNA bending and kinking. However, these proteins often display only a weak sequence preference, making the analysis of their DNA binding characteristics difficult if not impossible in a standard electrophoretic mobility assay (EMSA). In contrast, such proteins often bind prebent DNAs with high affinity and specificity. A synthetic cruciform DNA structure will often provide an ideal binding site for such proteins, allowing their affinities for both bent and linear DNAs to be directly and simply determined by a modified form of EMSA. © 2009 Humana Press, a part of Springer Science+Business Media, LLC.
CITATION STYLE
Stefanovsky, V. Y., & Moss, T. (2009). The cruciform DNA mobility shift assay: A tool to study proteins that recognize bent DNA. Methods in Molecular Biology, 543, 537–546. https://doi.org/10.1007/978-1-60327-015-1_31
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