Whole-mount in situ hybridization and immunohistochemistry in Xenopus Embryos

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Abstract

Xenopus is a favorable experimental model in developmental biology. With its fast and external development, high number of progeny and large size, early embryos are well suited for micromanipulation to study the function of genes with relevance to human diseases. In this chapter, we present a combined method for lineage tracing and whole-mount in situ hybridization. In addition, we present protocols for immunohistochemistry and assays to monitor the cell proliferation and apoptosis in whole embryos.

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Pera, E. M., Acosta, H., Gouignard, N., & Climent, M. (2015). Whole-mount in situ hybridization and immunohistochemistry in Xenopus Embryos. In In Situ Hybridization Methods (pp. 151–167). Springer New York. https://doi.org/10.1007/978-1-4939-2303-8_8

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