Tandem affinity purification (TAP) coupled to mass spectrometry has become a powerful approach to identify protein–protein interactions from different biological systems, including plants, in a proteome-wide manner. By using two sequential affinity purification steps, TAP allows for isolation of high-purity TAP-tagged proteins of interest and their associated proteins. Here we describe optimized procedures to use the GSRhino TAP technology for protein complex isolation from Arabidopsis cell suspension cultures.
CITATION STYLE
García-León, M., Iniesto, E., & Rubio, V. (2018). Tandem affinity purification of protein complexes from arabidopsis cell cultures. In Methods in Molecular Biology (Vol. 1794, pp. 297–309). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7871-7_21
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