Energy Transfer

Abstract

The book consists of 26 chapters. Each of these can be seen as a single part dealing with one topic. Every chapter ends with a list of literature references. Chapter 1 starts with the principles of fluorescence describing physical characteristics, for example emission, life-times, and quantum yields. The second chapter offers insights into instrumentation for fluorescence spectroscopy with an introduction to devices such as spectrofluorimeters, lightsources, filters, and detectors. Further on different applica- tions of fluorescence spectroscopy are described explicitly. The measurement methods and the information one can gain from these are explained equally well. The description covers experiments such as life-time measurements, fluo- rescence-quenching, and energy-transfer experiments, fluo- rescence correlation spectroscopy, and life-time imaging microscopy. For all these topics one can find basic information and useful practical hints. Hence this book covers all topics related to fluorescence spectroscopy, starting with basic information and moving toward actual topics of research and application. Valuable information is also given in chapters dedicated to fluorophores. In these chapters common natural fluorophores, and novel fluoro- phores such as lanthanides, quantum dots, and metal–ligand complexes are presented and their different properties are discussed. The book contains three appendices providing standard data and spectra in a clearly arranged overview. At the very end, answers to questions asked throughout the book are given and discussed. Another point to be stressed is the well selected, coloured figures which impart information precisely and in a straightforward manner.