Mesenchymal stem cells (MSCs) are multipotent cells that exhibit two main characteristics which define stem cells: self-renewal and differentiation. MSCs can migrate to sites of injury, inflammation, and tumor. Moreover, MSCs undergo myofibroblast-like differentiation, including increased production of α-SMA in response to transforming growth factor-β (TGF-β), a growth factor commonly secreted by tumor cells to evade immune surveillance. Based on our previous findings, hMSCs become activated and resemble carcinoma-associated myofibroblasts upon prolonged exposure to a conditioned medium from MDAMB231 human breast cancer cells. In this section, we show using immunofluorescence that keratinocyte-conditioned medium (KCM) induces differentiation of MSCs to resemble dermal myofibroblast-like cells with punctate vinculin staining and F-actin filaments.
CITATION STYLE
Mishra, P. J., & Banerjee, D. (2023). Visualizing Activated Myofibroblasts Resulting from Differentiation of Mesenchymal Stem Cells. In Methods in Molecular Biology (Vol. 2593, pp. 83–92). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2811-9_5
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