Synthesis of silver nanoparticles using α-NADPH-dependent nitrate reductase and phytochelatin in vitro has been demonstrated for the first time. The silver ions were reduced in the presence of nitrate reductase, leading to the formation of a stable silver hydrosol 10-25 nm diam. and stabilized by the capping peptide. The nanoparticles were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and UV-Vis absorption. These studies will help in designing a rational enzymatic strategy for the synthesis of nanomaterials of different chemical composition, shapes and sizes as well as their separation. © 2007 Springer Science+Business Media B.V.
CITATION STYLE
Kumar, S. A., Abyaneh, M. K., Gosavi, S. W., Kulkarni, S. K., Pasricha, R., Ahmad, A., & Khan, M. I. (2007). Nitrate reductase-mediated synthesis of silver nanoparticles from AgNO 3. Biotechnology Letters, 29(3), 439–445. https://doi.org/10.1007/s10529-006-9256-7
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