Expression of class II Chitinase gene in chilli (Capsicum annuum L.) as response to Fusarium oxysporum pathogen attack

Abstract

Chilli plants have many mechanism defenses to pathogen attack. The biotic stress can induce genes encoding Pathogenesis Related (PR) proteins to increase expression. The aim of this research is to analyze a molecular responds of CaChi2 gene as PR protein in chilli, which is infected by fungal pathogenic Fusarium oxysporum. Quantitative real-time polymerase chain reaction (qRTPCR) was used to measure the fluorescence and the ddCt method was used to analyze the expression level of CaChi2 in chilli. The result showed, up-regulated expression of resistant plants but no change in expression of susceptible plants after many range of time. It is concluded that the CaChi2 gene can be a molecular marker of resistance to Fusarium oxysporum in chilli.