DETAILED STUDY ON THE BLOOD CLOTTING BEHAVIOUR OF WOUND USING CHITOSAN, SODIUM ALGINATE AND CALCIUM ALGINATE SUBSTRATE

Abstract

The application of nanoparticle to textile material has been the object of several studies aimed at producing different functional performance. Nanoparticle research is currently an area of intense scientific research, due to a wide variety of potential applications in biomedical, optical, and electronic fields. Lot of research work has been done by using nano fibre technology to find a remedial solution for the problem faced in bio medial field. The outcome research shows that the nano technology can be effectively used in wound care, organ repair. Since the nano fibre got more surface area than the normal material it facilitates more adhesion of cells and drugs. The optimization of process parameters in the electrospinning process, we can get more fibre orientation which results in the enhancement in cell differentiation and drug release control. Biopolymers used in medical applications are alginate, chitosan, hyaluronic acid, collagen, polyurethane, polyethylene oxide, cellulose, gelatin. The production of chitosan nanofiber over polypropylene nonwoven is done by using electrospinning method. Then the coating of chitosan nanoparticle over chitosan nanofiber and a wound dressing material is developed. The effectiveness of the developed wound dressing material is characterized by blood clotting test sodium alginate, calcium alginate, chitosan. Further for characterization of nano particles is done by using FTIR, SEM, particle size distribution technique. From the research work it has been observed that the ball milling process considerably reduces the particle size of components in nano size particle. From SEM analysis we can conclude that after ball milling there is change in morphological structure. Chitosan has good blood clotting function when increasing the concentration of chitosan clotting time is reduced.