Haploid Seed Formation via Parthenogenesis in Bletilla

Abstract

This chapter provides a protocol to induce haploid plant formation in Bletilla via parthenogenesis by the application of 1-naphthaleneacetic acid sodium salt through the stigmatic surface of the column. Although the seed number is low, in vitro seed germination results in the formation of viable seedlings, and the ploidy levels as determined using flow-cytometric analyses indicate the formation of haploid and diploid (doubled haploid) plants. The successful induction of haploid plants through parthenogenesis can be a powerful tool in orchid breeding.