Embryo rescue and induction of somatic embryogenesis as a method to overcome seed inviability in Zea mays ssp. mays x Zea mays ssp. parviglumis crosses

7Citations
Citations of this article
8Readers
Mendeley users who have this article in their library.

Abstract

Zea mays ssp. mays (2n=40) and Z. mays ssp. parviglumis (2n=20) were crossed to obtain hybrid plants by embryo rescue. Hybrid embryos were isolated and cultured on García et al. (1992) basic medium supplemented with 2,4-dichlorophenoxyacetic acid and/or kinetin in different concentrations. Caryopses harvested 23 d after pollination (DAP) were turgid, with 0.3 to 0.5 mm long embryos, while those harvested 30 DAP were shrunken, with 1 to 1.5 mm long embryos. Twenty days after plating, 100% of the younger embryos gave rise to white, compact embryogenic calli. Subsequently, coleoptiles, leaf-like structures, shoots and roots originated from them and 35 hybrid plants were regenerated from 60 embryos. Embryogenic or organogenic calli frequencies did not differ among hormonal treatments, but they decreased, on average, from 90.5 to 44.3%, comparing 50 and 120-d-old cultures. The older embryos regenerated plants only by germination, although they gave rise to organogenic callus with low frequencies. Regenerated plants showed a somatic chromosome number of 2n=30, pollen fertility of 40 to 80% and 15% viable naked caryopses.

Cite

CITATION STYLE

APA

García, M. D., & Del, M. (2001). Embryo rescue and induction of somatic embryogenesis as a method to overcome seed inviability in Zea mays ssp. mays x Zea mays ssp. parviglumis crosses. Biologia Plantarum, 44(4), 497–501. https://doi.org/10.1023/A:1013718015315

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free