Preparation of colloidal gold particles and conjugation to protein A/G/l, IgG, F(ab′)2, and streptavidin

Abstract

Colloidal gold probes, including protein A/G/L, IgG, F(ab′)2, and streptavidin labeled with gold particles,are useful tools to localize antigens in cells and tissues by immunoelectron microscopy (IEM). This chapter describes different methods for the preparation of colloidal gold and conjugation of colloidal gold to protein A/G/L, IgG, and streptavidin.