Bioactive Components and Antioxidant Activity of Sargassum plagiophyllum Crude Extract

Abstract

Brown seaweed (Sargassum plagyophyllum) is known to contain natural antioxidants, namely flavonoids and phenolics. This study was aimed to determine the proximate composition of S. plagyophyllum, identify bioactive components, and antioxidant activity of the crude extracts of S. plagiophyllum. Bioactive components were extracted using several organic compounds including n-hexane, ethyl acetate, and methanol. The analysis parameters consisted of proximate analysis, yield, phytochemical, and antioxidant activity using the DPPH method. The results showed that the chemical composition of S. plagyophyllum seaweed were moisture 15.98 (ww), ash 21.38%, lipid 0.88%, protein 9.05%, carbohydrates 68.69% and crude fiber 22.24% (dw). The yield of extract with hexane, ethyl acetate, and methanol were 0.6, 0.28, and 0.31%, respectively. The hexane extract contained steroid/triterpenoid compounds while ethyl acetate extract contained alkaloids, flavonoids, steroids/ triterpenoids, saponins, and phenolics. The methanolic extract contained all bioactive components except flavonoids. Hexane, ethyl acetate and methanolic extract had antioxidant activity with IC50 values 1105.58 ppm, 532.42 ppm, and 777.79 ppm, respectively.