Aktivitas ampisilin terhadap bakteri MRSA setelah dikombinasi dengan uap minyak kayu manis menggunakan metode kontak

Abstract

Uncontrolled use of antibiotics in the community can have an impact on bacterial resistance to antibiotic therapy, one of which is the bacterium Methicillin-Resistant Staphylococcus aureus (MRSA). Essential oils have been used to treat infection and the vapor from it can be used as aromatherapy. One of the plants producing essential oil in Indonesia is cinnamon (Cinnamomum burmannii), which is known to have an antibacterial activity. The combination of antibiotic with essential oils can be an alternative to defeat bacterial resistance. Objective: This study aimed to determine the activity of ampicillin against MRSA bacteria after being combined with cinnamon essential oil. Method: The cinnamon bark simplicia was isolated using steam-water distillation to obtain volatile oil and was identified for the chemical components by GC-MS instrument. Antibiotic activity and its combination were determined by agar diffusion and gaseous contact method. Results: The results showed that the main compounds of cinnamon oil were cinnamaldehyde (64.84%), 1,8-cineole (6.4%), and benzyl benzoate (6.33%). The antibiotic activity of ampicillin alone against MRSA bacteria was 10.67 mm, and ampicillin combination with cinnamon oils vapor was 23.67 mm. Conclusion: The increased ampicillin activity after combined with cinnamon oil vapor was 121.84%. This suggests that the use of cinnamon essential oils is potential as a supportive therapy to treat MRSA infections.