Targeted insertional mutagenesis was used to construct hagA, hagB, and hagC hemagglutinin mutants of Porphyromonas gingivalis. pJRD215-derived plasmids containing tetA(Q)2 and portions of the targeted genes were conjugated into P. gingivalis. Interruption of the three loci was confirmed by Southern hybridization, sequencing, reverse transcription-PCR, and microtiter hemagglutination assays. No significant differences in hydrophobicity or coadherence to Actinomyces viscosus were detected between the mutants and the wild-type strain.
CITATION STYLE
Lépine, G., Ellen, R. P., & Progulske-Fox, A. (1996). Construction and preliminary characterization of three hemagglutinin mutants of Porphyromonas gingivalis. Infection and Immunity, 64(4), 1467–1472. https://doi.org/10.1128/iai.64.4.1467-1472.1996
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