Single-cell cytokine profiling to investigate cellular functional diversity in hematopoietic malignancies

Abstract

Single-cell analysis of cytokine production is increasingly recognized as an important method to understand the inflammatory microenvironment and hematopoietic disease state. Certain cytokines are critical to the regulation of lineage specification, and the aberrant production of these cytokines can contribute to lineage reprogramming. Here, we describe of a platform combining subnanoliter microchambers and a highdensity antibody barcode array for the study of single-cell cytokine secretions in hematopoietic cancer cell populations. Key words Single-cell cytokine profiling, Immunophenotyping, Functional heterogeneity, Highthroughput multiplexed analysis, Cytokine secretions, Pathogenesis