The quality of sperm post-immobilization at some parts of FH sperm using laser diodes

Abstract

The aim of this study was to find the proper method for sperms immobilization without reducing DNA integrity and damaging the shape of bovine sperms. Sperm immobilization was carried out by double shots using laser Octax MTG with wavelength of 1.48 μm and Eye Ware 3.0 software. A total of 20 sperms were shoot at 4 points, which was the tail end, the mid-tail, the neck and the head of the sperms. The shooting of 1.48 μm laser had the highest effect on immobility reaching 100% on the head and the DNA integrity of the sperms reached up to 90%, the shape of sperms was remained normal (100%) and did not showing significant effect. The results of this study indicated that (1) the laser diode with a wavelength of 1.48 μm at the mid-tail section of the sperm was most effective for sperm immobilization; (2) the use of laser diodes with a wavelength of 1.48 μm can be used to immobilize the sperms before intracytoplasmic sperm injection (ICSI). The diode laser shooting with a wavelength of 1.48 μm at the tail was the most effective in immobilizing the sperms with an immobility of 95.00 %5.98 and DNA integrity of 85.00% 11.65.