Tenascin-C expression in the lymph node pre-metastatic niche in muscle-invasive bladder cancer

25Citations
Citations of this article
11Readers
Mendeley users who have this article in their library.

Abstract

Background: Markers of stromal activation at future metastatic sites may have prognostic value and may allow clinicians to identify and abolish the pre-metastatic niche to prevent metastasis. In this study, we evaluate tenascin-C as a marker of pre-metastatic niche formation in bladder cancer patient lymph nodes. Methods: Tenascin-C expression in benign lymph nodes was compared between metastatic (n = 20) and non-metastatic (n = 27) patients with muscle-invasive bladder cancer. Urinary extracellular vesicle (EV) cytokine levels were measured with an antibody array to examine potential correlation with lymph node inflammation. The ability of bladder cancer EVs to activate primary bladder fibroblasts was assessed in vitro. Results: Lymph node tenascin-C expression was elevated in metastatic patients vs. non-metastatic patients, and high expression was associated with worse survival. Urinary EVs contained four cytokines that were positively correlated with lymph node tenascin-C expression. Bladder cancer EVs induced tenascin-C expression in fibroblasts in an NF-κB-dependent manner. Conclusions: Tenascin-C expression in regional lymph nodes may be a good predictor of bladder cancer metastasis and an appropriate imaging target. It may be possible to interrupt pre-metastatic niche formation by targeting EV-borne tumour cytokines or by targeting tenascin-C directly.

References Powered by Scopus

ImageJ2: ImageJ for the next generation of scientific image data

4285Citations
N/AReaders
Get full text

The nuclear factor NF-kappaB pathway in inflammation.

3918Citations
N/AReaders
Get full text

Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET

3063Citations
N/AReaders
Get full text

Cited by Powered by Scopus

The Functional Role of Extracellular Matrix Proteins in Cancer

116Citations
N/AReaders
Get full text

Immune determinants of the pre-metastatic niche

74Citations
N/AReaders
Get full text

Tumor-derived Cav-1 promotes pre-metastatic niche formation and lung metastasis in breast cancer

45Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Silvers, C. R., Messing, E. M., Miyamoto, H., & Lee, Y. F. (2021). Tenascin-C expression in the lymph node pre-metastatic niche in muscle-invasive bladder cancer. British Journal of Cancer, 125(10), 1399–1407. https://doi.org/10.1038/s41416-021-01554-z

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 4

80%

Researcher 1

20%

Readers' Discipline

Tooltip

Biochemistry, Genetics and Molecular Bi... 3

50%

Immunology and Microbiology 2

33%

Agricultural and Biological Sciences 1

17%

Save time finding and organizing research with Mendeley

Sign up for free