Electrophysiological recordings from neuroepithelial stem cells

Abstract

During embryonic development, neuroepithelial cells act as neural stem cells by renewing themselves. These cells are tightly interconnected and make contact with the basement membrane of the neuroepithelium. Under such circumstances, intracellular recording with a fine-tipped microelectrode is a successful method to study the electrophysiological properties of the neuroepithelial stem cell. This chapter describes the detailed techniques of intracellular recording from neuroepithelial stem cells.