Recent Advances in MS-Based Plant Proteomics: Proteomics Data Validation Through Integration with Other Classic and -Omics Approaches

Abstract

While reviewing the plant proteomics topic, both from a conceptual and methodological point of view and with a historical perspective, recent advances, current states, challenges, and future directions of the field are discussed. Proteomicsis moving, even though very slowly, from the descriptive era to a new era in which data start to be validated and integrated with other classic and -omics approaches, in the Systems Biology direction. This review is organized in different sec- tions, starting with a general and historical introduction, moving to platforms and techniques employed currently, data validation to confidently conclude from a biological point of view, workflow in a multi-omics experiment, highlighting some illustrative investigations, and finishing with some conclusions and remarks. As quite reviews have been already published in the field of mass spectrometry- based proteomics and to avoid being repetitive, this review only reports the most relevant contributions published recently by our research groups and those found in the literature.